About the Program


Objectives
- To comprehensively and accurately characterize the human perinatal microbiome throughout gestation
- To identify microbial etiologies of the great obstetrical syndromes, especially preterm labor with intact membranes and preterm prelabor rupture of membranes
- To train Maternal-Fetal Medicine and Postdoctoral Fellows in the latest molecular microbiologic techniques


Research Highlights
- Demonstrated that the human placenta does not have a microbial signal beyond that also evident in background technical controls in typical pregnancies
- Using non-human primate and mouse models, further demonstrated that the mammalian fetus is not colonized by microbial communities in-utero
- Characterized the molecular microbiology of the human upper genital tract outside pregnancy and demonstrated that the endometrium is not dominated by Lactobacillus spp.


Select Publications
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Lack of evidence for microbiota in the placental and fetal tissues of rhesus macaques. Theis KR, Romero R, Winters AD, Jobe AH, Gomez-Lopez N. mSphere. 2020; 5:e00210-20.
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No consistent evidence for microbiota in murine placental and fetal tissues. Theis KR, Romero R, Greenberg JM, Winters AD, Garcia-Flores V, Motomura K, Ahmad MM, Galaz J, Arenas-Hernandez M, Gomez-Lopez N. mSphere. 2020; 5:e00933-19.
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Microbial burden and inflammasome activation in amniotic fluid of patients with preterm prelabor rupture of membranes. Theis KR, Romero R, Motomura K, Galaz J, Winters AD, Pacora P, Miller D, Slutsky R, Florova V, Levenson D, Para R, Varrey A, Kacerovsky M, Hsu CD, Gomez-Lopez N. J Perinat Med. 2020 Feb 25; 48:115-131. PMID: 31927525
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Does the endometrial cavity have a molecular microbial signature? Winters AD, Romero R, Gervasi MT, Gomez-Lopez N, Tran MR, Garcia-Flores V, Pacora P, Jung E, Hassan SS, Hsu CD, Theis KR. Sci Rep. 2019;9:9905.
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Does the human placenta delivered at term have a microbiota? Results of cultivation, quantitative real-time PCR, 16S rRNA gene sequencing, and metagenomics. Theis KR, Romero R, Winters AD, Greenberg JM, Gomez-Lopez N, Alhousseini A, Bieda J, Maymon E, Pacora P, Fettweis JM, Buck GA, Jefferson KK, Strauss JF 3rd, Erez O, Hassan SS. Am J Obstet Gynecol 2019; 220:267.e1-267.e39.
Faculty